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Specifically, the whistleblowers believed that there were many more misclassified positions than were captured in the audit and that the agency significantly underreported the waste directly and tangentially associated with the widespread practice of misclassifying positions, Kerner wrote

This will prevent flow from entering the drainage tube, leaving a pathway from the flush port into the drainage bag

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After blocking with 5% non-fat milk or 5% BSA, blots were incubated with primary antibodies overnight at 4 C