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Overcoming mutation-based resistance to antiandrogens with rational drug design

Among them, capillary electrophoresis-mass spectrometry (CE-MS) combines the high resolution of capillary electrophoresis with the high sensitivity of mass spectrometry ( in situ spatial distribution of hormones such as insulin, glucagon, and their protein variants in human and mouse islets with high spatial resolution

In the reaction mixtures without enzyme, varying amounts of MBB (0-300 l of 1 mM stock solution which gave final concentration 0-100 M) were added into different fluorescent cuvettes already containing 3.0 ml of phosphate buffer (pH 6.5) and 100 l of GSH stock solution (3 mM)

Glutathione itself is synthesized intracellularly and is not absorbed systemically in significant amounts when taken orally, limiting the likelihood of competitive interactions

Govindan et al