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Simply put, the only safe amount of nicotine while pregnant is none

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The resulting cDNA was amplified using 2 SYBR mix (Qiagen) and 1 M of each primer in a StepOne Plus Thermocycler (Applied Biosystems) in Quantitative Reverse Transcriptase Polymerase Chain Reaction (qRT-PCR)

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Cigarettes are a frequent source of fires leading to loss of lives in private homes, which prompted both the European Union and the United States to ban cigarettes that are not fire-standard compliant from 2011 onwards