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To investigate whether the increased GSH level at 100 g/ml 5-FOA (Figure S2A) was stable during longer-term cultivation of the modified strain, cellular GSH content was monitored every 10 generations during sequential batch cultivation in flasks at 100 g/ml 5-FOA (after every ~ 10 generations, aliquots of overnight culture were transferred to fresh medium giving OD 0.01 and re-incubated)

[DOI] [PMC free article] [PubMed] [Google Scholar] 103.Doreddula S.K., Bonam S.R., Gaddam D.P., Desu B.S.R., RamaRao N., Pandy V

2023 Jun 27;15(6):e41020

Alfafara C, Kanda A, Shioi T, Shimizu H, Shioya S, Suga K (1992) Effect of amino acids on glutathione production by Saccharomyces cerevisiae

Glutathione is a powerful antioxidant your body naturally produces to help protect cells from damage