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A 2019 study by Perovic et al

After equilibration was reached, N -ethylmaleimide (NEM, final concentration 40 mM) or iodoacetamide (final concentration 50 mM) were added and a second measurement loop was started

Health Aging

At clinical doses under supervision, the most common side effects are mild nausea in the first week (typically resolves by day 7 to 10), injection site reactions (redness, minor swelling, localized soreness lasting 24 hours), and occasional transient fatigue or mild headache in the first few days