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The primary antibodies were rabbit anti-FGF5 antibody (Thermo Fisher Scientific, USA), goat anti-SRD5A1 antibody (Abcam, UK), rabbit anti-androgen receptor antibody (Abcam, UK), goat anti-DKK1 antibody (Abcam, UK), beta-catenin polyclonal antibody (Proteintech, USA), c-MYC polyclonal antibody (Proteintech, USA), and beta tubulin monoclonal antibody (Proteintech, USA)

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Combinative treatment of -elemene and cetuximab could induce ferroptosis of KRAS-mutant colorectal cancer cells (CRC) by iron-dependent ROS accumulation, GSH depletion, lipid peroxidation, upregulation of HO-1 and transferrin, and downregulation of negative regulatory proteins for ferroptosis (GPX4, SLC7A11, FTH1, glutaminase, and SLC40A1), thus inhibiting tumor growth and lymph node metastases in vivo ( Curcuma zedoaria [Zingiberaceae