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Acknowledgements This work was supported by project number 2020/39/B/NZ9/01894 from the National Science Centre (NCN), Poland and by the project number WZ/WB-II/2/2023 from the Ministry of Science and Higher Education.The authors are very grateful to Professor Shimshon Belkin, Dr Tal Elad, Dr Liat Moscovici and Dr Sharon Yagur-Kroll, Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel for providing bacteria strains

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In vitro cytotoxicity assays using SH-SY5Y (human neuroblastoma), Caco-2 (intestinal permeability), and hCMEC/D3 (human brain endothelial) cells demonstrated that nanoencapsulation significantly reduced the inherent cytotoxicity of free coumarin

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