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To clone the missing 5' portion of the MMP sequence, the adapter ASLinker (5'-PO 4 -CTG CAG AAA GCT TGG TGG ATC CTA-NH 2 -3') was ligated to single stranded cDNA as described [34]

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[78] Most say they feel addicted, and feel misery and disgust at their inability to quit (in surveys, 7191% regret having started, over 80% intend to quit, around 15% plan to quit within the next month)

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