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Perform 3 sets of 10 holds, twice daily

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Quantitative Reverse Transcriptase Polymerase Chain Reaction (qRT-PCR) for TGF-1 mRNA Total RNA was extracted from frozen lung tissues with TRIzol reagent (TaKaRa Biotechnology Co., Ltd., Dalian, China) and was reverse-transcribed using a reverse transcription kit (TaKaRa Biotechnology Co., Ltd., Dalian, China)

Therefore, we evaluated alterations in immune cell infiltration in the lungs of LPS-administered mice with or without GHK-Cu pretreatment