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Gels were transferred to PVDF (polyvinylidene difluoride) membranes followed by probing with primary antibody solutions (1:200 for IGF-1, 1:1000 for IRS-1, phospho-STAT3 and STAT3, and 1:500 for actin) overnight at 4 C

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The results showed a significant reduction in dendritic spine density in the neurons of the HACE group (Fig

GHK-Cu + MOTS-c Frequently investigated in: Metabolic-regulation research Cellular-energy and mitochondrial-signaling pathways Recovery-focused metabolic pathways Physiological-resilience research Healthy-aging investigations Research commonly explores how mitochondrial-support pathways may interact with regenerative and tissue-maintenance signaling mechanisms