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Briefly, rat striatal tissues were homogenized in ice-cold lysis buffer (50 mM Tris, 150 mM NaCl, 2 mM EDTA, 1% NP-40, 0.5% sodium deoxycholate, pH = 7.4, with protease inhibitor (Sigma-Aldrich)), and rotated for 1 h at 4 C

Rothfuss A, Schuetz P, Bochum S, Volm T, Eberhardt E, Kreienberg R, Vogel W, Speir G: Induced micronucleus frequencies in peripheral lymphocytes as a screening test for carriers of a BRCA1 mutation in breast cancer families

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