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After harvesting, cells were plated to a density of 6 0.5 10 5 cells/mL in six-well plates (Corning) and allowed to incubate for 72 h at 37C in a 5% (v/v) CO 2 atmosphere to a confluence of 90%

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In other words, the coexistence of necrotic and apoptotic markers inside the same cell shape a heterogeneous phenotype characterized by a mixture of suicide program activation 33

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Naturally present in every cell, it is a peptide molecule composed of three amino acids: glutamine, cysteine, and glycine