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GraphPad Prism software version 5.0a was used to plot the graphs and to calculate statistics

Because the continuous gradient is formed by a physical process, so long as the temperature and time are well controlled, the shape of the gradient is highly reproducible

Furthermore, to study the long-term antibody response and FoB function, we infected Gclc fl/fl and Gclc fl/fl Mb1-Cre + mice with lymphocytic choriomeningitis virus Clone 13 (LCMV Cl13), which causes a chronic infection 89,90,91,92,93

Telomeres are regions of repetitive DNA sequences at the ends of chromosomes that protect DNA health from damages

The mixture was incubated in a water bath at 100 C for 1 h, then cooled on ice for 45 min