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Laxa et al., 2019)

We note that the applicant's inclusion/exclusion criteria and reasoning for the third analysis are unclear

These are all based on clinical trials

Another assay developed showed that uric acid uptake applied to URAT1 overexpressing cells also indicated that 75 did not affect the transport activity of URAT1, the main target of current uricosuric drugs, revealing that 75 acted as a specific XO inhibitor with no direct effect on uric acid excretion

NO content was determined as the total value measured in the presence of cells minus the value determined from the media alone in the absence of cell growth, according to the manufacturer's protocol