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Briefly, cells were disrupted (Sonics Vibra Cell, Sonics & Materials Inc., Newtown, CT, USA) in an ice-cold mixture of methanol (1.66 ml) and chloroform (0.83 ml) for 3 minutes

J Biol Chem 2018, 293 (20), 75227530

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Characterization of mouse embryonic cells deficient in the Ctr1 high affinity transporter