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Blood samples were collected during the prehospital phase, and plasma GFAP levels were measured using the i-STAT Alinity point-of-care device

Following a 15-min incubation, samples were analyzed either on the Fortessa LSRII system using FACS Diva 6.1.1 software (BD Biosciences) or on the Symphony A3 system with FACS Diva v9.0 software (BD Biosciences)

We ended up referring in another family member who was also in need of rehabilitation

the receiving medium placed in the diffusion cells must be one which mimics the physiological condition of the skin

This model gives you the best of both worlds: speed and proper medical supervision