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10.1016/j.ijbiomac.2011.01.016 343 WangK.LinS.NuneK

It is also important to ascertain how long the depigmenting effect thus achieved would last and what the long-term effects would be on skin color by repetitive treatments lasting several weeks

Frequency depends on your individual goals and health condition

Slow, steady rotations

Primary antibody (mouse anti--tubulin or biotinylated anti--tubulin, both diluted to 1 g/mL in blocking buffer) was added to the blot holder and incubated