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When glutathione is involved in antioxidant reactions, it becomes the oxidized form, GSSG (glutathione disulfide)

To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled

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By adding a mixture of reduced and oxidized glutathione, your protein can sample different disulfide bond states and should end up in the correct native state, if that is the most energetically favorable (Figure 6)