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Autoantibodies against the angiotensin receptor AT1, particularly the second loop (AT1-AA) [210], can stimulate NAD(P)H oxidase, leading to increased generation of ROS

Proximity ligation assay (PLA) The Duolink In Situ Red Starter Kit Mouse/Rabbit was used for PLA, in accordance with the manufacturers instructions

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The receptor was originally isolated from a human gastric tumor cell line and contains a chain of 50 kDa and a chain of 145 kDa which are linked with disulphide bridges in a transmembrane complex of 190 kDa