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Digital engagement and enhanced outcomes A 2025 retrospective study found that digital engagement, including app-based tracking, coaching, and community support, significantly enhanced weight loss outcomes in adults treated with tirzepatide

RNA (200 ng) was reversely transcribed with First Strand cDNA Synthesis Kit (Thermo Scientific) containing oligo (dT)18 primer and reverse transcriptase

SIRT1 function is also modulated through protein-protein interactions: DBC1 inhibits SIRT1 activity via complex formation, 30,49 whereas AROS and SENP1 interact with SIRT1 to enhance its activity, expanding the spectrum of functional regulation

Then, which enhanced drought-induced proline accumulation, thus improving drought tolerance by regulating cellular redox potential (La et al., 2019)

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