[DOI] [PMC free article] [PubMed] [Google Scholar] [37].Wang B, Chen S, Zheng Q
DAY 02: CLAY LAMBADA VISIT We will get up early (04:30 in the morning) and have a light coffee or mate to start our 40 minutes trip to the Chuncho Macaw Clay Lick in the Bahuaja-Sonene National Park
Pyo HK, Yoo HG, Won CH, et al
The injection runs about 5 to 10 minutes
The sections were then incubated with primary antibodies against occludin (Abcam, dilution 1:100) and ZO-1 (ThermoFisher, dilution 1:100), diluted in PBS containing 0.2% BSA and 0.1% saponin at 4 C overnight, washed, and incubated for 1 h with Alexa Fluor 647 donkey anti-rabbit IgG secondary antibody (Invitrogen, dilution 1:400)