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MILK (Cattle): Zero (0) days

The bacterial cells were harvested, resuspended in binding buffer (20 mM sodium phosphate, pH 7.4, containing 500 mM NaCl and 20 mM imidazole), and lysed by treatment as above, followed by sonication

We did collect ExerVs from female subjects, and future studies will address this question

To minimize this risk, healthcare providers must inject into the upper outer quadrant of the buttock muscle, avoiding the nerves pathway