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In turn, these free radicals cause further mitochondrial damage and create a vicious cycle of less energy and more damage

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Immunoprecipitation The Pierce Classic Magnetic IP/Co-IP Kit (88804, Thermo Fisher Scientific) was used for immunoprecipitation

Specifically, GHK-Cu was found to be least possible to cause skin irritation, while CuCl 2 and Cu(OAc) 2 were found to induce the expression of various skin irritation biomarkers, i.e., IL-1, IL-8, FOSL1, and HSPA1A