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Most patients self-inject with an insulin-gauge needle the injection takes 30 seconds and is virtually painless

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Further, a near perfect correlation between mRFP-p-actin labeled spines and the general presynaptic marker Synapsin and VLGUT1 staining, which identifies excitatory glutamatergic synapses suggests that most AnglV-dependent effects on hippocampal spines were restricted to excitatory synapses

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