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Ethics statement The animal study was approved by Institutional Committee for the Care and Use of Animals at Nanjing Medical University

In contrast to earlier assumptions, this generation of protein adducts can take place before GSH levels are depleted extensively [15,23] and also after therapeutic doses of APAP without relevant GSH depletion [15,16]

FIGURE 4 3.6 Effect of cigarette smoke exposure and ASA treatment on glt-1, xct, and nf-b mRNA expression in the NAc Cigarette smoke exposure for 31 days decreased relative glt-1 mRNA expression in the NAc and this decrease was reversed by 15 mg/kg and 30 mg/kg ASA treatment (Figure 5A)