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The gene encoding AMFR-E2G2 (fusion of the RING domain of human AMFR (residues 227-298) and human E2G2 (residues 1-165)) was previously cloned into the pET28a-SUMO expression vector 51

10.1177/153537020422900206 8 AndersonM

Phorbol ester stimulates a protein kinase C-mediated agatoxin-TK-sensitive calcium permeability pathway in human red blood cells

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The regulatory disulfide bond of the plant GCL has a midpoint potential comparable with other known redox-responsive plant proteins (Hicks et al., 2007)