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Evolutionarily conserved and nonconserved cellular localizations and functions of human SIRT proteins

Brcic, I

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The resulting acyl-phosphate intermediate undergoes nucleophilic attack by the -amino group of cysteine, which may be activated by an active site glutamate

Data were acquired in data-independent acquisition PASEF mode with the following settings: m / z range from 100 m / z to 1,700 m / z , ion mobility range from 1/K 0 = 1.300.85 V s cm 2 using equal ion accumulation and ramp times in the dual TIMS analyser of 100 ms each