Western blotting The protein extracts of the lung tissues and cells were prepared using Radio Immunoprecipitation Assay (RIPA) buffer (Phygene, China) with Phenylmethanesulfonyl fluoride (PMSF) (Solarbio, China), Next, the protein concentrations were tested by a protein quantification kit (BCA Assay) (Zomanbio, China)
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