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The unlabeled fluorophores were separated from the labeled protein solution using a resin column (provided in the kit) equilibrated previously with 1 PBS elution buffer (0.1 M potassium phosphate, 1.5 M NaCl, pH 7.2, with 2 mM sodium azide)
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Trained as a ballet dancer, Swayze brought a rare physical grace to his performances, which helped define iconic roles that combined toughness with vulnerability
Les taux de composs chimiques dans les arosols d'e-cigarettes sont gnralement moindre qu'avec la fume de tabac, mais une quantit importante de propylne glycol (PG) vaporis, de glycrol vgtale (VG), de nicotine et de substances toxiques, telles que les aldhydes et des mtaux lourds, a t rapporte [159]
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