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Supplementary Material ACKNOWLEDGEMENTS We thank Peter Selby and his Nicotine Dependence Services team at Centre for Addiction and Mental Health, Toronto, Canada for their assistance in funding acquisition, reviewing protocol of the review, and knowledge transition

As each part of the body is attacked the risk of disease is obvious with an estimated 1 in 2 smokers dying from a smoking related disease

All the healthy subjects did not undergo any antibiotic treatment for 1 month prior to the sample collection

Parasites are an inescapable presence in modern horses