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The culture conditions of isolated strains were as follows: LB medium (5.0 g/L yeast extract, 10.0 g/L tryptone, and 10.0 g/L NaCl, pH 7.0, sterilized at 115 for 30 min), potato dextrose agar (PDA) medium (200.0 g/L potato was cut into small pieces, boiled for 2030 min, and filtered with 8 layers of gauze), and glucose 20.0 g/L, agar 20.0 g/L, natural pH

This in turn affects the livelihood and health of fishing communities in coastal areas, and those who eat seafood affected by the contamination

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Keeping it in longer wont boost effect, it only increases dryness and irritation

Full bodied, hints of coffee and cream, made at the Esteli factory in Nicaragua