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Next, it was centrifuged at 13000 rpm for 10 min and washed three times with DI water to obtain PDA

A procedure called Kasai hepatoportoenterostomy (KPE) that replaces the obliterated extrahepatic bile duct with an intestinal conduit is the first-line treatment to re-establish the bile flow with only 50% success rate ( BA is most likely a clinical phenotype resulting from a number of prenatal or perinatal insults to the hepatobiliary tree, and this disease is underpinned by a number of key pathobiological factors (KPFs) either singly or more often in combinations

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