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CRC cells increase glutamine metabolism to maintain TCA cycle activity when glucose metabolism decreases, leading to higher levels of amino acids, especially aspartic acid and asparagine

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10.1080/19490976.2020.1734423 159 WuZ.ZhangB.ChenF.XiaR.ZhuD.ChenB.et al (2023)

Third, while bulk proteomics provides a comprehensive view of cellular differentiation pattern, more precise methods such as single-cell technologies (transcriptomics or proteomics) 38,39 and spatiotemporal single-cell tracking 40,41 would further elucidate the detailed cellular composition and 3D organization during iHLO development