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For example, significant reductions in yield or cured leaf quality can have serious ramifications for growers. His group uses classical breeding by intentional crossing, molecular marker-assisted breeding, and plant transformation to study tobacco gene function

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doi:10.1186/s13256-019-2215-4

[119] By the mid-17th century every major civilization had been introduced to tobacco smoking and in many cases had already assimilated it into its culture, despite the attempts of many rulers to stamp the practice out with harsh penalties or fines

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