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Western Blot Analysis Cellular and cerebral samples were lysed in RIPA (Beyotime, China) containing inhibitor cocktail (Roche, Germany) for 20 min, following which the lysates were centrifuged (12,000 g) at 4C for 15 min
[DOI] [PubMed] [Google Scholar] 22.Huang C., Wang Y., Li X., Ren L., Zhao J., Hu Y., Zhang L., Fan G., Xu J., Gu X., et al
Once NRF2 has been activated, a cascade of events occur that induce expression of much higher levels of the bodys natural anti-oxidant defenses
This pathway activation enables: Enhanced neuronal survival under stress conditions Increased dendritic arborization and synaptic density Improved axonal sprouting and regeneration Activation of neuronal repair mechanisms Studies using neuronal cell cultures revealed that Cerebrolysin significantly increases neurite outgrowth and protects against excitotoxic injury, effects attributed to neurotrophic factor-like activities[2]
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