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With Puff Bars being marketed as disposable and single-use, this gives buyers an misguided idea of how to dispose of their Puff Bars, and the wrong disposal of its materials can lead to detrimental effects on the environment

Other tissue explants were placed on coverslips (2 explants on each of coverslips with 22 22mm in size), which were placed into 6-well (3 cm in diameter)-BD Falcon culture plates (Becton Dickinson) containing FCS medium, and incubated and treated, as described above

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