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The standard diagnostic pathway includes a serum B12 blood test as the initial screening tool

Neither is approved for human use by the FDA or any other major regulatory body, and the findings below come from laboratory and animal studies

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Prior to mitochondrial isolation, 50-200 L of Pierce Anti-HA magnetic beads (VWR, PI88837) were washed in KPBS (136 mM KCl [Sigma-Aldrich, P5405], 10 mM KH 2 PO 4 , pH 7.4 in HPLC grade H 2 O [Fisher Chemical, W5-1]) for each sample