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Huh7-Lunet-T7 RC cells (human hepatocellular carcinoma cells expressing dengue reporter construct) were maintained at 37 C with 5% CO 2 in DMEM containing 10% FCS, GlutaMax, 1% ( v/v ) nonessential amino acids, 100 g/mL penicillin-streptomycin, 1 g/ml Puromycin and 5 g/ml Zeocin
During such reactions, transient species containing unpaired electron(s) are formed
The ideal frequency really depends on your individual health needs and how your body responds
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