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Limitations of current studies When considering the limitations, not all studies used the same level of microscopic assessment, however, this is to be expected

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Since Ca 2+ influx through L-type VSCCs is one of the main sources of intracellular Ca2 +, hippocampal neurons were pre-treated with manidipine, a blocker of voltage-gated L-type VSCCs, to assess the role of L-type VSCCs in this process

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Due to the comparative small size of the rats and large size of the defects, these defects would fairly mimic large fistulas in patients that would hardly heal spontaneously