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MCF7, A549, and HT-1080 cells were cultured in RPMI 1640 medium from WelGENE supplemented with 10% FBS and 1% penicillin-streptomycin

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Similar to our discussion in the FY 2022 IPPS/LTCH PPS proposed rule (86 FR 25340), and the FY 2023 IPPS/LTCH PPS proposed rule (87 FR 28290), we note that while TERLIVAZ may address an unmet need because it is the first treatment indicated specifically for the treatment of HRS, the applicant's assertion that TERLIVAZ does not involve the treatment of the same/similar type of disease and the same/similar patient population when compared to an existing technology, on the basis that there is a subset of patients for whom current treatments are ineffective and for whom TERLIVAZ will offer a new treatment option, does not necessarily speak to the treatment of a new patient population for HRS

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