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Additionally, treatment retention is a critical aspect, with dropout rates varying significantly based on the type of programranging from 20-70% for inpatient programs and 23-50% for outpatient programs

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Briefly, DE cells in the second 50-ml conical tube were resuspended in Hepatic Specification Medium, composed of Hepatocyte Differentiation Basal Medium supplemented with 100 ng/ml HGF, 1% DMSO as well as 10 M Y-27632 and seeded onto Matrigel pre-coated 12-well or 24-well plates, at a seeding density of 8x104 cells/cm2

Obesity impacts not only the plasma concentration of HDLC but also its functionality (Wang & Peng, 2011)