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As a champion free radical scavenger, it assists in metabolizing nutrients, regulating gene expressions, and protecting against an array of age-related and pathogenic disorders

The assay was performed by heating samples in a Mx3005p qPCR machine (Agilent Technologies, USA) from 25 to 97 C in 1 C min 1 increments for 30 s in an expanding saw-tooth profile, a refinement of a previously described protocol 24

Chen L, Hambright WS, Na R, Ran Q (2015) Ablation of the ferroptosis inhibitor glutathione peroxidase 4 in neurons results in rapid motor neuron degeneration and paralysis

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Pelleted bacterial cells were lysed in 30 ml of lysis buffer (20 mM Tris-Cl (pH 7.5), 0.1% Triton X-100, 1 mg/ml lysozyme) for 20 min and sonicated, and insoluble molecules were removed