Clinical studies used vastly different protocols
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Just one of the many reasons the body stores vitamin B12 is because it is necessary for the proper development of red blood cells, which are essential in reducing the risk of conditions such as pernicious anemia and other ailments that can lead to further complications
The cells were washed once in with 50 L PBS (Gibco, #14190-094) after which 30 L of each concentration from the dilution series was added to the 384-well assay plate with cells
4.2 Detailed expandable studies Study 1: Lau 2021, pivotal Phase 2 monotherapy Citation: Lau D.C.W., Erichsen L., Francisco A.M., et al