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Neurochemical parameters determination Calorimetric assay kits (Biodiagnostic Co., Egypt) were used to measure malondialdehyde (MDA), hydrogen peroxide (H 2 O 2 ), nitric oxide (NO), and glutathione reduced (GSH) in brain cortical tissue from all groups

These inflammatory responses not only impair testicular tissue, but also directly affect key cells involved in spermatogenesis, including spermatogonia, primary spermatocytes, and Sertoli cells, thereby exacerbating spermatogenesis disorders and leading to reduced sperm count and quality [67, 277]

Exercise: Regular resistance and aerobic training naturally upregulates NAD+ biosynthesis and mitochondrial adaptation

Brown CW, Amante JJ, Chhoy P, Elaimy AL, Liu H, Zhu LJ, et al

The final composition should be 10% w/v SLU-PP-332, 30% v/v PEG 400, 15% v/v ethanol, and 45% v/v buffered aqueous phase